Arrange the following steps of applying immersion oil in the correct sequence chegg

1. Focus at low power on a region of a smeared and stained specimen which is well-spread and stained (not too thin, nor too thick).

2. Rotate turret to 40x objective, locate desired portion of specimen in the center of the field. Refocus very carefully so that the specimen is focused as sharply as possible. (Do not alter focus for the following steps )

3. CRITICAL STEP:

Partially rotate turret so that 40x and 100x objectives straddle the specimen.

Place a small drop of oil on the slide in the center of the lighted area. (Take care not to dribble on the stage.)

Note the small drop of oil directly over the area of the specimen to be examined.

4. Rotate turret so that the 100x oil immersion objective touches the oil and clicks into place.

Focus only with fine focus. Hopefully, the specimen will come into focus easily. Do not change focus dramatically. If you still have trouble, move the slide slightly left and right, looking for movement in the visual field, and focus on the object which moved.

5. With more than one specimen on a slide, do not alter focusing, rather, place a drop of oil on the second specimen, and slide the slide laterally until it is in place.

6. Never go back to the 10x or 40x objectives after you have applied oil to the specimen since oil can ruin the lower power objectives. [The 4x objective can be used because it is high enough to be above the oil.]

7. Clean up!: When you have finished for the day, wipe the 100x oil immersion objective carefully with lens paper to remove all oil. Wipe oil from the slide thoroughly with a Kimwipe. Cleanse stage should any oil have spilled on it. Recap the immersion oil container securely, replace in drawer.