When it is required that a specimen must be kept warm, at which temperature must it be kept?

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Terms in this set (43)

Which one of the following would be considered a preexamination/preanalytical error?

Under filling the aliquot container

Which blood specimen would be chilled during a 45-minute transport?

Blood gases

Which blood specimen should be kept warm during transport to the laboratory?

Cold agglutinins

Which of the following analyses should be protected from light?

Porphyrins

Which analyte is photosensitive?

Bilirubin

Which of the following practices will likely cause hemolysis?

Shaking the tube to mix the contents

After blood collection, when should plasma be centrifuged?

As soon as possible

Serum should be removed from blood cells after which time period?

120 minutes

A specimen should be protected from light for which of the following determinations?

Bilirubin concentration

A specimen should be chilled for which of the following analyses if there is a significant deliver delay?

Complete blood count

A portion of a blood sample that has been removed/separated from the primary specimen tube.

Aliquot

A laboratory result that indicates a pathophysiologic state at such variance with normal as to be life threatening; these values should be defined and reported to the patient's physician as soon as possible. Also called the "panic value."

Critical laboratory value

Sensitivity to light; photosensitive substances will decompose after exposure to bright light. Ex: bilirubin

Photosensitive

Transportation system used in many health care facilities for specimens and paper-based documentation. Considerations for use of these systems involves evaluation of speed, distance, control mechanisms, shock absorbency, sizes of carriers, and breakage/ spillage rates.

Pneumatic tube system

Describes a substance that is sensitive to higher temperatures; specimens being tested for these substances need to be chilled immediately.

Thermolabile

The time it takes for a blood specimen to be ordered, collected, transported, processed, analyzed, and a result reported.

Turnaround time (TAT)

An emergency or critical situation that requires immediate action; in the case of blood collection and analysis. Should be given the highest priority for collection, delivery to lab, analysis, and reporting.

STAT

What are two specimens that need chilling?

1. Ammonia
2. Lactic Acid

What are 3 specimens that require heating?

1. Cold agglutinins
2. Cryofribinogen
3. Cryoglobulins

What are two specimens that are photosensitive?

1. Bilirubin
2. Vitamin A

Made by immune system when infection is present the RBC's will clump together at low temperatures.

Cold Agglutinins

Abnormal proteins.

Cryoglobulins

What are 3 methods of delivery?

1. Hand delivery
2. Pneumatic Tube system
3. Automated carrier

Require guidelines for timeliness, may have log sheet for specimens, may have special transport containers.

Hand delivery

Systems that propels cylindrical containers through a network of tubes by compressed air.

Pneumatic Tube System

Requires more room than tube system. Consists of small container car attached to a network of tracks that is routed to appropriate site, such as lab or nursing station. Usually over head in ceiling tiles.

Automated carrier

Regulates and monitors specimen and biological shipments in U.S.

Department of Transportation

Established guidelines to promote safety and security for shipping biohazardous specimens.

CDC

Can cause permanent disability , life threatening, or a fatal disease if exposed by healthy person, animal. Exposure occurs when infectious substance is released from protective package, physical contact is then made.

Category A

Will not usually cause permanent disability or life threatening, or fatal if exposed to it. Substances are being transported for diagnostic or investigation.

Category B

Material that is suspected of containing a pathogen.

Infectious substances

What are three specimens that do not need to be centrifuged?

Hematology test:
1. RBC
2. Reticulocyte counts
3. Platelets counts

How specimen is handled after drawing the blood and before it goes into the centrifuge.

Precentrifugation

(with anticoagulants), centrifuged immediately.

Plasma specimens

(without anticoagulants) need 30-60 minutes to
clot at room temperature.

Serum specimens

Causes specimen to rotate at high speeds, causes heavier elements to sink to the bottom of tube, lighter elements stay on top of the tube. (time and speed are critical)

Centrifugation

After centrifugation, prior to removing serum/plasma from cells.

Postcentrifugation

If testing is not completed within in 48 hours, what should you do with the specimen?

Freeze it

_______________ and __________ state that lab results should be confirmed, dated, have a permanent report and be on patients permanent medical record.

Joint commission and CAP

Also states reports include patient ID, date and hour test was completed and be signed off by lab personnel doing procedure.

CAP

This type of report is done for STAT results and critical values.

Verbal report

Most common is horizontal and fixed, allows for spinning gel tubes in a horizontal rotor, will have a flat gel barrier, preferable for chemistry testing.

Rotor centrifuge

Revolutions per minute (RPM)

Spin time

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Which specimen should be transported at 37 degrees Celsius?

Cryoglobulin is a common and simple clinical immunology laboratory test that requires the blood samples to be transported to the laboratory, without cooling, at 37°C (1, 5, 6).

How can specimens be maintained at 37 C during transport and handling?

Specimens that must maintain at 37 degrees C during transport and handling should be warmed in a heel warmer (only last 30 minutes) before and after collection. Some tests require warming of the sample in a 37 Degree C incubator before testing.

Which sample must be kept warm until it is delivered to the laboratory for separation?

Final Exam Study Questions.

What is a Thermolabile specimen?

Thermolabile refers to a substance which is subject to destruction, decomposition, or change in response to heat. This term is often used to describe biochemical substances. For example, many bacterial exotoxins are thermolabile and can be easily inactivated by the application of moderate heat.