Which of the following identifies a major mechanism of electricity-induced injury?

The term that identifies how close a set of results are to each other when running a quality control validation or similar study is called:

This staining mechanism is known to be dependent upon the presence of nucleic acids (DNA or RNA) that form salt-type unions.

Staining with basic (positively charged dyes) dyes

To aid in diagnosis, it is recommended that LIVER biopsy samples are sectioned at what micrometer (μm) thickness?

A technologist pipetted 200 microliters of serum in a test tube and added 5 milliliters of saline to the tube. What is the dilution ratio of serum to the total volume in the tube?

There is 200 microliters of serum added to 5000 microliters of saline, for a total volume of 5200 microliters. 200 microliters of the total 5200 microliters is a dilution equaling 1:26.

Which of the following is found in the Alcian blue solution:

The bevel angle is the angle of the

-angle of the very tip of a blade (cannot be adjusted by the knife tilt on a microtome)

between the block face and lower facet of the knife. It is dependent upon the tilt or knife holder and is usually between 3-8°. This is the angle that we are most concerned with and it can be adjusted easily to suit the blade and block for better sectioning. Once the proper clearance angle is found for a particular blade, it rarely needs to be changed.

The four main classes of connective tissue are:

blood, bone, cartilage, and connective tissue

When should an avidin-biotin blocking step be performed?

Only when staining tissues that are most likely to contain endogenous biotin

With this special stain liposarcomas can be differentiated, as well as fat emboli, and muscle diseases.

should be discarded after 2 or 3 days

In the ATPase method at pH 9.4-10.4, how will type I muscle fibers appear?

highly formalin resistant and do not require retrieval.

Which of the following identifies a major mechanism of electricity-induced injury?

  • Electrical energy causing direct tissue damage
  • Conversion of electrical energy into thermal energy causing massive tissue destruction
  • Direct trauma resulting from falls or violent muscle spasms

Formal calcium is recommended especially for use in the fixation and preservation of

Some examples of bluing solutions include

ammonia water, dilute lithium carbonate, and Scott's tap water

Which category of fixative works by altering the tertiary structure of tissue proteins?

Non-coagulant fixatives act by

creating a gel of surface proteins that makes subsequent penetration of reagents more difficult

The van Gieson solution stains collagen:

The van Gieson solution stains muscle:

What can SV40 monoclonal antibody be used to detect?

A represent in the diagram

Tissue size and tissue type should dictate the amount of time required for tissue to properly fix and process. Turn-around time should not be the

driving force behind tissue fixation and processing

Tissue should not exceed what thickness in order to obtain optimal routine tissue processing results?

3 mm will produce the best slides

All of the examples provided will cause a "parched Earth" effect

-The use of a fluorocarbon spray to chill the block.
-The flotation bath temperature being too hot.
-The tissue is not properly processed.

Electrical injury is damage caused by generated electrical current passing through the body. Symptoms range from skin burns to damage to internal organs and other soft tissues, cardiac arrhythmias, and respiratory arrest. Diagnosis is based on history, clinical criteria, and selective laboratory testing. Treatment is supportive, with aggressive care for severe injuries.

Although accidental electrical injuries encountered in the home (eg, touching an electrical outlet or getting shocked by a small appliance) rarely result in significant injury or sequelae, accidental exposure to high voltage results in nearly 300 deaths annually in the US. There are > 30,000 nonfatal shock incidents a year in the US, and electrical burns account for about 5% of admissions to burn units in the US.

Traditional teaching is that the severity of electrical injury depends on Kouwenhoven’s factors:

  • Type of current (direct [DC] or alternating [AC])

  • Voltage and amperage (measures of current strength)

  • Duration of exposure (longer exposure increases injury severity)

  • Body resistance

  • Pathway of current (which determines the specific tissue damaged)

However, electrical field strength, a newer concept, seems to predict injury severity more accurately.

AC changes direction frequently; it is the current usually supplied by household electrical outlets in the US and Europe. DC flows in the same direction constantly; it is the current supplied by batteries. Defibrillators and cardioverters usually deliver DC current. How AC affects the body depends largely on frequency. Low-frequency (50- to 60-hertz [Hz]) AC is used in US (60 Hz) and European (50 Hz) households. Because low-frequency AC causes extended muscle contraction (tetany), which may freeze the hand to the current’s source and prolong exposure, it can be more dangerous than high-frequency AC and is 3 to 5 times more dangerous than DC of the same voltage and amperage. DC exposure is likely to cause a single convulsive contraction, which often throws the person away from the current’s source.

For both AC and DC, the higher the voltage (V) and amperage (A), the greater the ensuing electrical injury (for the same duration of exposure). Household current in the US is 110 V (standard electrical outlet) to 220 V (used for large appliances, eg, refrigerator, dryer). High-voltage (> 500 V) currents tend to cause deep burns Burns Burns are injuries of skin or other tissue caused by thermal, radiation, chemical, or electrical contact. Burns are classified by depth (superficial and deep partial-thickness, and full-thickness)... read more

Which of the following identifies a major mechanism of electricity-induced injury?
, and low-voltage (110 to 220 V) currents tend to cause muscle tetany and freezing contact to the current’s source. The maximum amperage that can cause flexors of the arm to contract but that allows release of the hand from the current’s source is called the let-go current. Let-go current varies with weight and muscle mass. For an average 70-kg man, let-go current is about 75 milliamperes (mA) for DC and about 15 mA for AC.

Tissue damage due to electrical exposure is caused primarily by the conversion of electric energy to heat, resulting in thermal injury. Amount of dissipated heat energy equals amperage2 × resistance × time; thus, for any given current and duration, tissue with the highest resistance tends to suffer the most damage. Body resistance (measured in ohms/cm2) is provided primarily by the skin, because all internal tissue (except bone) has negligible resistance. Skin thickness and dryness increase resistance; dry, well-keratinized, intact skin averages 20,000 to 30,000 ohms/cm2. For a thickly calloused palm or sole, resistance may be 2 to 3 million ohms/cm2; in contrast, moist, thin skin has a resistance of about 500 ohms/cm2. Resistance for punctured skin (eg, cut, abrasion, needle puncture) or moist mucous membranes (eg, mouth, rectum, vagina) may be as low as 200 to 300 ohms/cm2.

If skin resistance is high, more electrical energy may be dissipated at the skin, resulting in large skin burns but less internal damage. If skin resistance is low, skin burns are less extensive or absent, and more electrical energy is transmitted to internal structures. Thus, the absence of external burns does not predict the absence of electrical injury, and the severity of external burns does not predict the severity of electrical injury.

Damage to internal tissues depends on their resistance as well as on current density (current per unit area; energy is concentrated when the same current flows through a smaller area). For example, as electrical energy flows in an arm (primarily through lower-resistance tissues, eg, muscle, vessels, nerves), current density increases at joints because a significant proportion of the joint’s cross-sectional area consists of higher-resistance tissues (eg, bone, tendon), which decreases the area of lower-resistance tissue; thus, damage to the lower-resistance tissues tends to be most severe at joints.

Electrical field strength is the intensity of electricity across the area to which it is applied. It, along with Kouwenhoven’s factors Kouwenhoven’s factors Electrical injury is damage caused by generated electrical current passing through the body. Symptoms range from skin burns to damage to internal organs and other soft tissues, cardiac arrhythmias... read more , also determines the degree of tissue injury. For instance, 20,000 volts (20 kV) distributed across the body of a man who is about 2 m (6 ft) tall result in a field strength of about 10 kV/m. Similarly, 110 volts, if applied only to 1 cm (eg, across a young child’s lip), result in a similar field strength of 11 kV/m; this relationship is why such a low-voltage injury can cause the same severity of tissue injury as some high-voltage injuries applied to a larger area. Conversely, when considering voltage rather than electrical field strength, minor or trivial electrical injuries technically could be classified as high voltage. For example, the shock received from shuffling across a carpet in the winter involves thousands of volts but causes inconsequential injury.

The electrical field effect can cause cell membrane damage (electroporation) even when the energy is insufficient to cause any thermal damage.

Application of low electrical field strength causes an immediate, unpleasant feeling (being “shocked”) but seldom results in serious or permanent injury. Application of high electrical field strength causes thermal or electrochemical damage to internal tissues. Damage may include

  • Hemolysis

  • Protein coagulation

  • Coagulation necrosis of muscle and other tissues

  • Thrombosis

  • Dehydration

  • Muscle and tendon avulsion

High electrical field strength injuries may result in massive edema, which, as blood in veins coagulates and muscles swell, can result in compartment syndrome Compartment Syndrome Compartment syndrome is increased tissue pressure within a closed fascial space, resulting in tissue ischemia. The earliest symptom is pain out of proportion to the severity of injury. Diagnosis... read more . Massive edema may also cause hypovolemia and hypotension. Muscle destruction can result in rhabdomyolysis Rhabdomyolysis Rhabdomyolysis is a clinical syndrome involving the breakdown of skeletal muscle tissue. Symptoms and signs include muscle weakness, myalgias, and reddish-brown urine, although this triad is... read more and myoglobinuria, and electrolyte disturbances. Myoglobinuria, hypovolemia, and hypotension increase risk of acute kidney injury Acute Kidney Injury (AKI) Acute kidney injury is a rapid decrease in renal function over days to weeks, causing an accumulation of nitrogenous products in the blood (azotemia) with or without reduction in amount of urine... read more . The consequences of organ dysfunction do not always correlate with the amount of tissue destroyed (eg, ventricular fibrillation Ventricular Fibrillation (VF) Ventricular fibrillation causes uncoordinated quivering of the ventricle with no useful contractions. It causes immediate syncope and death within minutes. Treatment is with cardiopulmonary... read more

Which of the following identifies a major mechanism of electricity-induced injury?
may occur with relatively little tissue destruction).

Symptoms and Signs of Electrical Injuries

Burns Burns Burns are injuries of skin or other tissue caused by thermal, radiation, chemical, or electrical contact. Burns are classified by depth (superficial and deep partial-thickness, and full-thickness)... read more

Which of the following identifies a major mechanism of electricity-induced injury?
may be sharply demarcated on the skin even when current penetrates irregularly into deeper tissues. Severe involuntary muscular contractions Dystonias Dystonias are sustained involuntary muscle contractions of antagonistic muscle groups in the same body part, leading to sustained abnormal posturing or jerky, twisting, intermittent spasms that... read more , seizures Seizure Disorders A seizure is an abnormal, unregulated electrical discharge that occurs within the brain’s cortical gray matter and transiently interrupts normal brain function. A seizure typically causes altered... read more , ventricular fibrillation Overview of Arrhythmias The normal heart beats in a regular, coordinated way because electrical impulses generated and spread by myocytes with unique electrical properties trigger a sequence of organized myocardial... read more
Which of the following identifies a major mechanism of electricity-induced injury?
, or respiratory arrest Overview of Respiratory Arrest Respiratory arrest and cardiac arrest are distinct, but inevitably if untreated, one leads to the other. (See also Respiratory Failure, Dyspnea, and Hypoxia.) Interruption of pulmonary gas exchange... read more due to central nervous system (CNS) damage or muscle paralysis may occur. Brain, spinal cord, and peripheral nerve damage may result in various neurologic deficits. Cardiac arrest Cardiac Arrest Cardiac arrest is the cessation of cardiac mechanical activity resulting in the absence of circulating blood flow. Cardiac arrest stops blood from flowing to vital organs, depriving them of... read more may occur in the absence of burns as in bathtub accidents (when a wet [grounded] person contacts a 110-V circuit—eg, from a hair dryer or radio).

Young children who bite or suck on extension cords can burn their mouth and lips. Such burns may cause cosmetic deformities and impair growth of the teeth, mandible, and maxilla. Labial artery hemorrhage, which results when the eschar separates 5 to 10 days after injury, occurs in up to 10% of these young children.

An electrical shock can cause powerful muscle contractions or falls (eg, from a ladder or roof), resulting in dislocations Overview of Dislocations A dislocation is complete separation of the 2 bones that form a joint. Subluxation is partial separation. Often, a dislocated joint remains dislocated until reduced (realigned) by a clinician... read more (electrical shock is one of the few causes of posterior shoulder dislocation Shoulder Dislocations In shoulder (glenohumeral) dislocations, the humeral head separates from the glenoid fossa; displacement is usually anterior. Shoulder dislocations account for about half of major joint dislocations... read more

Which of the following identifies a major mechanism of electricity-induced injury?
), vertebral Overview of Fractures A fracture is a break in a bone. Most fractures result from a single, significant force applied to normal bone. In addition to fractures, musculoskeletal injuries include Joint dislocations... read more
Which of the following identifies a major mechanism of electricity-induced injury?
or other fractures Overview of Fractures A fracture is a break in a bone. Most fractures result from a single, significant force applied to normal bone. In addition to fractures, musculoskeletal injuries include Joint dislocations... read more
Which of the following identifies a major mechanism of electricity-induced injury?
, injuries to internal organs, and other blunt force injuries.

Symptoms and signs reference

  • 1. Wesner ML, Hickie J: Long-term sequelae of electrical injury. Can Fam Physician 59(9):935-939, 2013.

  • Head-to-toe examination

  • Sometimes ECG, cardiac enzyme measurement, and urinalysis

Asymptomatic patients who are not pregnant, have no known heart disorders, and who have had only brief exposure to household current usually have no significant acute internal or external injuries and do not require testing or monitoring. For other patients, ECG, complete blood count (CBC), measurement of cardiac enzymes, and urinalysis (to check for myoglobin) should be considered. Patients with impaired consciousness may require CT or MRI.

  • Shutting off current

  • Resuscitation

  • Analgesia

  • Sometimes cardiac monitoring for 6 to 12 hours

  • Wound care

The first priority is to break contact between the patient and the current source by shutting off the current (eg, by throwing a circuit breaker or switch, by disconnecting the device from its electrical outlet). High- and low-voltage power lines are not always easily differentiated, particularly outdoors. CAUTION: To avoid shock, rescuers should not try to disengage anyone entangled in power lines (whether of high or low voltage) until the power is shut off.

Patients are resuscitated while being assessed. Shock Prognosis Shock is a state of organ hypoperfusion with resultant cellular dysfunction and death. Mechanisms may involve decreased circulating volume, decreased cardiac output, and vasodilation, sometimes... read more , which may result from trauma or massive burns, is treated. Standard burn fluid resuscitation formulas, which are based on the extent of skin burns, may underestimate the fluid requirement in electrical burns; thus, such formulas are not used. Instead, fluids are titrated to maintain adequate urine output (about 100 mL/h in adults and 1.5 mL/kg/h in children). For myoglobinuria, maintaining adequate urine output is particularly important, while alkalinizing the urine may help decrease the risk of renal failure Acute Kidney Injury (AKI) Acute kidney injury is a rapid decrease in renal function over days to weeks, causing an accumulation of nitrogenous products in the blood (azotemia) with or without reduction in amount of urine... read more . Surgical debridement of large amounts of muscle tissue may also help to decrease myoglobinuric renal failure.

Severe pain due to an electrical burn is treated by the judicious titration of IV opioids.

Asymptomatic patients who are not pregnant, have no known heart disorders, and who have had only brief exposure to household current usually have no significant acute internal or external injuries that would necessitate admission and can be discharged.

Cardiac monitoring for 6 to 12 hours is indicated for patients with the following conditions:

  • Any suggestion of cardiac damage

  • Known heart disorders (possibly)

All patients with significant electrical burns should be referred to a specialized burn unit. Young children with lip burns should be referred to a pediatric orthodontist or oral surgeon familiar with such injuries.

Electrical devices that touch or may be touched by the body should be properly insulated, grounded, and incorporated into circuits containing protective circuit-breaking equipment. Ground-fault circuit breakers, which trip when as little as 5 milliamperes (mA) of current leaks to ground, are effective and readily available. Outlet guards reduce risk in homes with infants or young children.

To avoid injury from current that jumps (arcing injury), poles and ladders should not be used near high-voltage power lines.

  • In addition to burn injuries, AC can freeze the patient's hand to the current source, while DC can throw the patient, causing injury.

  • Although skin burn severity does not predict the degree of internal damage, internal damage is more severe if the skin has low resistance.

  • Examine patients completely, including for traumatic injuries.

  • Consider ECG, CBC, cardiac enzymes, urinalysis, and monitoring unless patients are asymptomatic, are not pregnant, have no known heart disorders, and have had only brief exposure to household current.

  • Refer patients with significant electrical burns to a specialized burn unit and, if significant internal damage is suspected, begin fluid resuscitation.

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What is a major mechanism of electricity

The major mechanisms of electricity-induced injury include: Electrical energy causing direct tissue damage. Conversion of electrical energy into thermal energy causing massive tissue destruction. Direct trauma resulting from falls or violent muscle spasms.

What are the 4 main types of electrical injuries?

There are four main types of electrical injuries: flash, flame, lightning, and true. Flash injuries, caused by an arc flash, are typically associated with superficial burns, as no electrical current travels past the skin.

What is the most common electrical injury?

Electrical burns are the most common shock-related, nonfatal injury. They occur when a worker contacts energized electrical wiring or equipment. Although electrical burns can occur anywhere on the body, they most often occur on the hands and feet.

What injuries are associated with electric shock?

Electric current can cause injury in four ways:.
Cardiac arrest due to the electrical effect on the heart..
Muscle, nerve, and tissue destruction from a current passing through the body..
Thermal burns from contact with the electrical source..
Falling or injury after contact with electricity..