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Which of the following is a list of the materials required for PCR?In general, a complete PCR reaction requires five basic PCR reagents; DNA/RNA template, DNA polymerase, primers (forward and reverse), deoxynucleotide triphosphates (dNTPs) and PCR buffers.
What is not necessary for the PCR quizlet?Which of the following molecules is not required for a PCR reaction? Ligase is not required for a PCR reaction. The enzyme used during PCR is a thermostable DNA polymerase. The thermostability of Taq polymerase is required during the annealing phase of PCR.
What is the most important advantage of PFU polymerase over Taq polymerase?What is the most important advantage of Pfu polymerase over Taq polymerase? Unlike Taq polymerase, Pfu polymerase has proofreading activity. Unlike Taq polymerase, Pfu polymerase functions well at relatively high temperatures.
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Which of the following statements is true regarding the use of oligonucleotide primers in PCR?
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